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The genus Aeromonas Stanier 1943 (Martin-Carnahan and Joseph, 2005) is a Gammaproteobacteria (Proteobacteria, Bacteria) that comprises a group of Gram-negative, rod-shaped bacteria, which are autochthonous to aquatic environments worldwide and are usual microbiota (as well as primary or secondary pathogens) of fish, amphibians and other animals (Janda and Abbott, 2010). We used the divergence time of Escherichia coli and Salmonella enterica as the calibration point. Molecular dating and macro evolutionary birth–death models were used to determine the temporal pattern of lineage diversification and significant changes in diversification rates were estimated using models with constant and variable diversification rates (Rabosky, 2006). Explosive evolutionary radiations: decreasing speciation or increasing extinction through time? doi: 10.1111/j.1558-5646.2008.00409.x Pub Med Abstract | Cross Ref Full Text | Google Scholar Retchless, A.
This approach has been regularly challenged by results obtained using datasets from a variety of organisms, ranging from bacteria to primates, which show considerable departures from clocklike evolution and constant rate variation among lineages, and it has become clear that the strict molecular clock hypothesis is not biologically realistic (Drummond et al., 2006). doi: 10.1186/1471-2180-12-62 Pub Med Abstract | Cross Ref Full Text | Google Scholar Sanderson, M.
When building a tree, every reconstruction method gives a branch length (bl), which is a function of the rate of substitution (μ) and the time of evolution (t): bl = μt. doi: 10.1016/0169-5347(96)81059-7 Cross Ref Full Text | Google Scholar Sanglas, A., Albarral, V., Farfán, M., Lorén, J.